LC-MS and GC-MS based metabolomics platform for cancer research
نویسندگان
چکیده
We present here a LC-MS and GC-MS based analytical platform for the comprehensive analysis of cellular metabolites, including water-soluble metabolites, and waterinsoluble fatty acids, and phospholipids. The entire workflow consists of metabolic extraction, LC-MS and GC-MS runs, data analysis and interpretation. Standard operation procedures have been developed for the metabolite extraction from cell culture, tissue and serum/ plasma, which involve liquid extraction using appropriate extraction solvents. Metabolites were analyzed using multiple analytical methods on multiple dedicated instruments. Cationic water-soluble metabolites were analyzed on a triple quadrupole instrument using hydrophilic interaction chromatography [1]. Anionic water-soluble metabolites were analyzed using high resolution ‘Exactive’ Orbitrap mass spectrometer coupled to reversed phase ion pairing chromatography [2]. Fatty acids and phospholipids were analyzed using Agilent Q-TOF instrument coupled to reversed phase chromatography [3]. Data analyses were performed using MAVEN program which converts the raw data into a validated table of metabolite-specific signals [4]. Examples will be provided to demonstrate our capability to analyze a broad range of metabolites from real biological samples, as well as to probe the metabolic fluxes using stable isotope tracers.
منابع مشابه
Metabolomics, a Powerful Tool for Agricultural Research
Metabolomics, which is based mainly on nuclear magnetic resonance (NMR), gas-chromatography (GC) or liquid-chromatography (LC) coupled to mass spectrometry (MS) analytical technologies to systematically acquire the qualitative and quantitative information of low-molecular-mass endogenous metabolites, provides a direct snapshot of the physiological condition in biological samples. As complements...
متن کاملMetabolic Profiling of Plasma from Benign and Malignant Pulmonary Nodules Patients Using Mass Spectrometry-Based Metabolomics
Solitary pulmonary nodule (SPN or coin lesion) is a mass in the lung and can be commonly found in chest X-rays or computerized tomography (CT) scans. However, despite the advancement of imaging technologies, it is still difficult to distinguish malignant cancer from benign SPNs. Here we investigated the metabolic profiling of patients with benign and malignant pulmonary nodules. A combination o...
متن کاملEnhanced Metabolite Profi ling from Bark of Alangium Salviifolium Using LC/MS and GC/Q-TOF Techniques
Traditional herbal remedies are used as alternative medicines for a number of diseases. Alangium salviifolium is one such plant, used as traditional medicinal plant. Several investigations have been performed using this plant extract to demonstrate its therapeutic value. However, very few attempts have been made to identify the extensive metabolite composition of this plant. In this Application...
متن کاملIntegrated Transcriptomics and Metabolomics Study of Retinoblastoma Using Agilent Microarrays and LC/MS/GC/MS Platforms
متن کامل
MUSCLE: automated multi-objective evolutionary optimization of targeted LC-MS/MS analysis
Developing liquid chromatography tandem mass spectrometry (LC-MS/MS) analyses of (bio)chemicals is both time consuming and challenging, largely because of the large number of LC and MS instrument parameters that need to be optimized. This bottleneck significantly impedes our ability to establish new (bio)analytical methods in fields such as pharmacology, metabolomics and pesticide research. We ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره 2 شماره
صفحات -
تاریخ انتشار 2014